INFORMAZIONI SU
CAPEZZALI Eleonora
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Supervisore: Prof.ssa Frossi
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| Despite huge advances in modern oncology, colorectal cancer (CRC) remains one of the most aggressive forms of cancer for which new therapeutic approaches are needed. Our project is to uncover and describe novel mechanisms involved in immune resistance, with a focus on B cells and mast cells (MCs), because their role in tumorigenesis and progression of CRC is largely obscure, although their impact has been documented. Therefore, taking advantage of CRC organoids derived from mice and patients, and single-cell RNA-sequencing (scRNA-seq), the intent of this PhD project is to perform whole transcriptome sequencing and CITE-seq for dissection of immune infiltrate in TME of CRC with a special eye on B cells and MCs. The specific goal of the project is to identify dysregulated pathways and differentially expressed surface-molecules as a potential target of innovative therapies, in the context of repolarization of the pro-tumoral immune-infiltrating cells into anti-tumoral. As the role of B cells and MCs in CRC TME is not well known, its characterization with a single-cell approach wishes to have a translational role in the design of new personalized pharmacological therapies for the treatment of this diffuse and lethal malignancy. |
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